General Information

  • ID:  hor006312
  • Uniprot ID:  Q8CHK2
  • Protein name:  Relaxin-3 B chain
  • Gene name:  Rln3
  • Organism:  Mus musculus (Mouse)
  • Family:  Insulin family
  • Source:  animal
  • Expression:  High expression in the brain localized to the pons/medulla with highest levels in pars ventromedialis of the dorsal tegmental nucleus. Significant expression is also detected in the spleen, thymus, lung, testis and ovary.
  • Disease:  NA
  • Comments:  NA
  • Taxonomy:   Mus (subgenus), Mus (genus), Murinae (subfamily), Muridae (family), Muroidea , Myomorpha (suborder), Rodentia (order), Glires , Euarchontoglires (superorder), Boreoeutheria , Eutheria , Theria , Mammalia (class), Amniota , Tetrapoda , Dipnotetrapodomorpha , Sarcopterygii (superclass), Euteleostomi , Teleostomi , Gnathostomata , Vertebrata , Craniata (subphylum), Chordata (phylum), Deuterostomia , Bilateria , Eumetazoa , Metazoa (kingdom), Opisthokonta , Eukaryota (superkingdom),cellular organisms
  • GO MF:  GO:0001664 G protein-coupled receptor binding; GO:0005179 hormone activity
  • GO BP:  GO:0007165 signal transduction
  • GO CC:  GO:0005576 extracellular region

Sequence Information

  • Sequence:  RPAPYGVKLCGREFIRAVIFTCGGSRW
  • Length:  27(25-51)
  • Propeptide:  MAMLGLLLLASWALLGALGLQAEARPAPYGVKLCGREFIRAVIFTCGGSRWRRADILAHESLGDFFADGEANTDHLASELDEAVGSSEWLALTKSPQAFYGGRASWQGSPGVVRGSRDVLAGLSSSCCEWGCSKSQISSLC
  • Signal peptide:  MAMLGLLLLASWALLGALGLQAEA
  • Modification:  NA
  • Glycosylation:  NA
  • Mutagenesis:  NA

Activity

  • Function:  May play a role in neuropeptide signaling processes. Ligand for LGR7, relaxin-3 receptor-1 and relaxin-3 receptor-2 (By similarity).
  • Mechanism:  NA
  • Cross BBB:  NA
  • Target:  Rxfp3, Rxfp1
  • Target Unid:  Q8BGE9, Q6R6I7
  • IC50: NA
  • EC50: NA
  • ED50: NA
  • kd: NA
  • Half life: NA

Structure

  • Disulfide bond:  NA
  • Structure ID:  AF-Q8CHK2-F1(AlphaFold_DB_ID)

  • Structure: (PDB_ID-from https://www.rcsb.org/; AlphaFold_DB_ID-from https://alphafold.ebi.ac.uk/; hordbxxxxxx_AF2.pdb was predicted structure by AlphaFold2; hordbxxxxxx_ESM.pdb was predicted structure by ESMFold)
    •    hor006312_AF2.pdbhor006312_ESM.pdb

Physical Information

Mass: 350453 Formula: C138H215N41O33S2
Absent amino acids: DHMNQ Common amino acids: GR
pI: 10.45 Basic residues: 5
Polar residues: 9 Hydrophobic residues: 10
Hydrophobicity: 5.56 Boman Index: -3708
Half-Life: 1 hour Half-Life Yeast: 2 min
Half-Life E.Coli: 2 min Aliphatic Index 72.22
Instability Index: 5659.26 Extinction Coefficient cystines: 7115
Absorbance 280nm: 273.65

Literature

  • PubMed ID:  12686464
  • Title:  Production of recombinant human relaxin 3 in AtT20 cells.
  • PubMed ID:  11689565
  • Title:  Human relaxin gene 3 (H3) and the equivalent mouse relaxin (M3) gene. Novel members of the relaxin peptide family.